human breast cancer cell line hcc70  (ATCC)

Journal: Heliyon

Article Title: Antitumor effect of a novel humanized MUC1 antibody-drug conjugate on triple-negative breast cancer

doi: 10.1016/j.heliyon.2023.e15164

Figure Lengend Snippet: HzMUC1 antibody binds to MUC1 on the cell surface of TNBC cells. a. Lysates from BT20, MB-468, HCC70, BT549, Hs578T, SUM149PT, and MB-231 cell lines were resolved by SDS-PAGE and immunoblotted with the commercially available anti-MUC1 C-terminal (CT) antibody and re-probed with anti-tubulin as a loading control. b. Cell lysates from HCC70, BT20, and Hs578T cells were immunoprecipitated with human IgG or HzMUC1 antibody and immunoblotted with the anti-MUC1-CT antibody. c. The HzMUC1 antibody binds MUC1 protein on the cell surface. HCC70 and Hs578T cells grown on coverslips were washed with PBS, incubated with HzMUC1 antibody (2 μg/well) on ice, and subsequently incubated with Alexa Fluor 488-conjugated secondary antibody. Nuclei were stained with Hoechst 33258. Images were visualized by confocal microscopy. Scale bars, 20 μm.

Article Snippet: Cell culture Human triple-negative breast cancer cell lines HCC70, BT-20, MB-468, BT549, Hs578T, SUM149PT, and MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: SDS Page, Control, Immunoprecipitation, Incubation, Staining, Confocal Microscopy

Journal: Heliyon

Article Title: Antitumor effect of a novel humanized MUC1 antibody-drug conjugate on triple-negative breast cancer

doi: 10.1016/j.heliyon.2023.e15164

Figure Lengend Snippet: Effects of MMAE conjugation on the binding capacity of HzMUC1 antibodies. A 96-well tissue culture plate was seeded with HCC70 cells. After 3 days, the cells were incubated with various concentrations (1, 10, 100, 100 or 10000 ng/mL) of human IgG antibodies, HzMUC1 antibodies and HzMUC1-MMAE. The binding ability of the HzMUC1 antibody or HzMUC1-MMAE to MUC1 was measured using ELISAs.

Article Snippet: Cell culture Human triple-negative breast cancer cell lines HCC70, BT-20, MB-468, BT549, Hs578T, SUM149PT, and MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Conjugation Assay, Binding Assay, Incubation

Journal: Heliyon

Article Title: Antitumor effect of a novel humanized MUC1 antibody-drug conjugate on triple-negative breast cancer

doi: 10.1016/j.heliyon.2023.e15164

Figure Lengend Snippet: HzMUC1-MMAE inhibits the growth of TNBC cells. HCC70 (a), BT20 (b), and Hs578T (c) cells were plated at low cell density and incubated with the indicated concentrations of HzMUC1-MMAE for 5 days. Cell growth was determined by staining the plates with crystal violet and quantified by measuring the absorbance at 540 nm. Percent cell growth is expressed relative to non-HzMUC1-MMAE treatment set to 100. IC50 values are indicated. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cell culture Human triple-negative breast cancer cell lines HCC70, BT-20, MB-468, BT549, Hs578T, SUM149PT, and MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, Staining

Journal: Heliyon

Article Title: Antitumor effect of a novel humanized MUC1 antibody-drug conjugate on triple-negative breast cancer

doi: 10.1016/j.heliyon.2023.e15164

Figure Lengend Snippet: HzMUC1-MMAE induces apoptosis in TNBC cells. a. HCC70, BT20, and Hs578T cells were treated with HzMUC1-MMAE (10, 20, or 40 nM) for 48 h. Cells were stained with annexin V and propidium iodide and analyzed by flow cytometry using NovoExpress software. b. Quantification of the flow cytometry data from a. Mean values from three experiments are shown. The HzMUC1-MMAE group was compared with the control group. **P < 0.01, ***P < 0.001. c. HCC70, BT20, and Hs578T cells treated with the indicated concentrations of HzMUC1-MMAE for 48 h were immunoblotted with anti-cleaved PARP antibodies and re-probed with β-actin as loading control.

Article Snippet: Cell culture Human triple-negative breast cancer cell lines HCC70, BT-20, MB-468, BT549, Hs578T, SUM149PT, and MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Flow Cytometry, Software, Control

Journal: Heliyon

Article Title: Antitumor effect of a novel humanized MUC1 antibody-drug conjugate on triple-negative breast cancer

doi: 10.1016/j.heliyon.2023.e15164

Figure Lengend Snippet: Therapeutic effects of HzMUC1-MMAE against TNBC tumor growth in a xenograft mouse model. A mouse xenograft model was established by implantation of HCC70 cells in BALB/c nu/nu mice. PBS or HzMUC1-MMAE (5 mg/kg) was injected intravenously into tumor-bearing mice when the tumors reached 7.5 mm in diameter. Tumor growth was monitored for 18 days (n = 5 each). Control mice injected with PBS are shown as a control (n = 5). a. Individual body weights for each treatment group. b. Tumor volume (width2 x length/2). Data are shown as mean ± standard deviation.*P < 0.05, **P < 0.01. c. Macroscopic appearance of dissected tumors from mice euthanized at the end of the experiment. d. Individual tumor weights. Mean values are indicated as a horizontal bar. Data are shown as mean ± standard deviation. **P < 0.01.

Article Snippet: Cell culture Human triple-negative breast cancer cell lines HCC70, BT-20, MB-468, BT549, Hs578T, SUM149PT, and MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Injection, Control, Standard Deviation

Journal: Heliyon

Article Title: Anti-metastatic action of an N 4 -aryl substituted thiosemicarbazone on advanced triple negative breast cancer.

doi: 10.1016/j.heliyon.2020.e05161

Figure Lengend Snippet: T2 reduces cell viability in triple negative breast cancer cell lines and induces apoptosis. (A) T2 formula. (B) Concentration log-response curve: results from MTS viability assays examining the effects of T2 on the growth of 4T1, LM38-LP and HCC70 cell lines. Cells were incubated with T2 or DMSO (control) for 5 days. (C) Cell apoptosis determined by Annexin V-FITC and propidium iodide staining. Flow cytometer analysis of the apoptotic and necrotic cells after 24 h of incubation with T2 (2.5, 5 and 10 μM) or DMSO (control). Results are expressed as mean ± standard deviation, ∗p < 0.05 (n = 3). (D) Western blots illustrating PARP cleavage, caspase-3 activation and Bcl-xL induction after T2 (2.5, 5 and 10 μM) or DMSO (control) treatment for 24 h. A representative blot of three is shown. (E) Densitometric analyses of three independent western blots.

Article Snippet: The HCC70 human breast cancer cell line, derived from a primary ductal triple negative carcinoma and obtained from ATCC, was cultured in RPMI medium supplemented with 10% FBS.

Techniques: Concentration Assay, Incubation, Control, Staining, Flow Cytometry, Standard Deviation, Western Blot, Activation Assay